Olympus FLUOVIEW FV1000 User Manual page 336

APPLIED OPERATIONS
4 Regions Mode
[Histogram] Graph
You can draw a pictorial figure
on [Histogram], using graphic
button located at lower right side
of dialog box.
See 2-12 Entering Comment in
Image for methods to delete
graphic, to select plural numbers
of graphics and to change color
of graphic frame.
[Annotation mode] Group
Box
When < Regions > option
button is selected, this dialog
box will appear.
[Annotation] Text Box
For details, refer to the explanation
that appears in next page.
<Accept Annotation> button
It extracts brightness only of image
designated in rectangular on [Histogram]
graph and creates new image window and
displays the image.
Original image
Colocalization image
OPERATION INSTRUCTIONS
Page
2 - 2 5 2
/Image Processing
Specifies the arbitrary region in [Histogram], and measures colocalization within the region.
In addition, distribution level of brightness in draw pictorial figure is displayed on image
window. Color is the same as color of draw pictorial figure selected.
Fig. 2-99 [Colocalization Processor] Dialog Box
In case of Annotation Mode is [Regions]
<Rectangular> Button
Rectangular draw: On graph,
drag diagonally from upper left
to lower right to draw
rectangular region.
<Circle> Button
Circle/Oval draw: Drag
diagonally rectangle that
circumscribes region of the
circle to be drawn on the
image.
<Poly region> Button
Polygon draw: Click each peak
to draw a polygon. When the
last peak is clicked, the peak
clicked at first time will be
connected with the last peak.
<Free Region> Button
Free draw: Specify region by
dragging on image. Releasing
the mouse button at the end of
drag, end and start positions
are connected.