Olympus FLUOVIEW FV1000 User Manual page 267

Setting Track 3
In XYT observation, return the zoom ratio to 2X and acquire 30 images every 5 seconds.
(Observe the change in fluorescence image with time after bleaching.)
1. Select "Series mode" in [Mode] of [Track].
2. Select "Normal" in [Start by] of [Track].
3. Enter "30" to [Condition] of [Track].
4. Enter "5" to [Frame Interval].
5. Enter "2" to [Zoom].
6. Select "Middle" in [Scan Speed]. (Return to the setting before bleaching.)
7. Enter the laser intensity value for image acquisition to [Laser Intensity].
8. Enter the PMT value of each channel to [PMT Voltage].
9. Enter the image file name to be stored to [Save FileName].
10. Enter a comment in [Comment] if necessary.
APPLIED OPERATIONS
OPERATION INSTRUCTIONS
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