Olympus FLUOVIEW FV1000 User Manual page 242

APPLIED OPERATIONS
OPERATION INSTRUCTIONS
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/ Protocol processor
Scan speed: Sets image acquisition speed from "Fast", "Middle", "Slow" and "Init" in the
pull-down menu.
The above speed is the same with those of [Scan Speed] group box in
[Acquire] panel. In case that [Fast] or [Fast Clip] is selected in [Sub mode],
[Scan Speed] is automatically set to [High Speed].
Laser Intensity: Enter the intensity setting for each laser. The setting is 0 to 100, and the
unit is %. When it sets to 0, a laser shutter is closed.
REX Mask: Select the REX mask file from the pull-down list whether to use the REX mask
which is displayed on FLUOVIEW software or to disable the mask by selecting
"None".
[REX Mask] appears only when AOTF (FV5-COMBA) is equipped.
PMT Voltage: Enter the PMT voltage of each channel. The setting is 1 to 1100, and the unit
is V.
Gain: Enter the gain of each channel. The setting is 1.0 to 10.0.
Offset: Enter the offset of each channel. The setting is 0 to 100, the unit is %.
C.A.: Enter the confocal aperture diameter for each channel. The setting is 50 to 800, the
unit is micron.
XY Stage Track No: When multi-point time lapse software is used, track number of multi-
point time lapse software that meets to this track will be displayed.
When multi-point time lapse software is not used, the display does not
appear.
Zstatus: Enter Z stage information.
Start Pos: Enter the Z-scanning start position of cross section of which observation is
required.
Stop Pos: Enter the Z-scanning stop position of cross section of which observation is
required.
Step Size: Enter the number of steps in Z direction.
Slices: Enter the number of image slices to be acquired
Save FileName: Input file name for acquired image. Image would be saved under path of
[Image File Name:].