Olympus FLUOVIEW FV1000 User Manual page 333

Confocal laser scanning biological microscope
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A
B
C
D
[Histogram] graph
Explanation of [Annotation] Text Box
Display in Text Box
Threshold Annotation
Total samples in histogram:
(Dye-name1)[Thresh:
<threshold:
>=threshold:
(Dye-name2)[Thresh:
<threshold:
>=threshold:
Upper-left
Samples
[of (Dye-name1) < threshold]
[of (Dye-name2) >= threshold]
Of all samples
Upper-right
Samples
[of (Dye-name1) >= threshold]
[of (Dye-name2) >= threshold]
Of all samples
Lower-left
Samples
[of (Dye-name1) < threshold]
[of (Dye-name2) < threshold]
Of all samples
Lower-right
Samples
[of (Dye-name1) >= threshold]
[of (Dye-name2) < threshold]
Of all samples
APPLIED OPERATIONS
Explanation
Thresholds Mode
Total numbers of image pixels to be processed
]
Name of Dye method. Threshold value in X-
axis.
Total numbers of image pixels below the
threshold value in X-axis
((A+C)/(A+B+C+D))x100 [%]
Total numbers of image pixels above the
threshold value in X-
axis((B+D)/(A+B+C+D))x100 [%]
]
Name of Dye method. Threshold value in Y-axis
Total numbers of image pixels below the
threshold value in Y-axis
((C+D)/(A+B+C+D))x100 [%]
Total numbers of image pixels above the
threshold value in Y-axis
((A+B)/(A+B+C+D))x100 [%]
Upper left portion of [histogram] graph
Total numbers of image pixels contained in
upper left portion
(A/(A+C))x100 [%]
(A/(A+B))x100 [%]
(A/(A+B+C+D))x100 [%]
Upper right portion of [histogram] graph
Total numbers of image pixels contained in
upper right portion
(B/(B+D))x100 [%]
(B/(A+B))x100 [%]
(B/(A+B+C+D))x100 [%]
Lower left portion of [histogram] graph
Total numbers of image pixels contained in
lower left portion
(C/(A+C))x100 [%]
(C/(C+D))x100 [%]
(C/(A+B+C+D))x100 [%]
Lower right portion of [histogram] graph
Total numbers of image pixels contained in
lower right portion
(D/(B+D))x100 [%]
(D/(C+D))x100 [%]
(D/(A+B+C+D))x100 [%]
/Image Processing
OPERATION INSTRUCTIONS
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