Olympus FLUOVIEW FV1000 User Manual page 276

Confocal laser scanning biological microscope
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APPLIED OPERATIONS
Setting Track 3
Setting Track 4
OPERATION INSTRUCTIONS
Page
2 - 1 9 2
/ Protocol processor
Acquire nine images at one-hour interval in each Track in XYT observation.
1.
Select "New mode"-"XYT" in [Mode] of [Track].
2.
Enter "Wait 3600" in [Stand by] of [Track]. (Enter the value by the second.)
3.
Enter "9" in [Condition] of [Track].
4.
Select "Middle" in [Scan Speed].
5.
Enter the laser intensity value for use in image acquisition in the cell for each laser in
[Laser Intensity].
Enter "8" in [Ar] and "50" in [HeNe-G].
6.
Enter the PMT value of each channel in the cell for each channel in [PMT Voltage].
Enter "Init" in [PMT2] and [PMT3].
7.
Enter the image file name to be stored to [Save FileName.].
Specifies the repeated protocol by use N variable.
1.
Select "Next" in [Mode] of [Track].
2.
It is not necessary to enter the values in other cells.

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