Olympus FLUOVIEW FV1000 User Manual page 334

APPLIED OPERATIONS
3 Min-Max Bounds Mode
[Histogram] Graph
Rectangle is displayed in graph.
The rectangular can be
positioned by dragging.
[Annotation] Text Box
For details, refer to the explanation
that appears in next page.
<Accept Annotation> button
It extracts brightness only of image
designated in rectangular on [Histogram]
graph and creates new image window and
displays the image.
Original image
Colocalization image
OPERATION INSTRUCTIONS
Page
2 - 2 5 0
/Image Processing
Specifies the rectangular on [Histogram] graph, and measures colocalization by use of the
rectangular.
In addition, distribution level of brightness inside rectangular is displayed on image window.
Color is the same as color of rectangular selected.
Fig. 2-98 [Colocalization Processor] Dialog Box
In case of Annotation Mode is [Min-Max Bounds]
[Annotation mode] Group
Box
When < Min-Max Bounds >
option button is selected,
this dialog will appear.
[Min.] Text Box
[Max.] Text Box
Max. and Min. Positions of
rectangle on [Histogram]
graph will be displayed.
You can input the values
directly and change the
values.
The value entered will be
automatically changed to
the value that can be
processed in software.
If the same value is entered
in both [Min.] and [Max.]
text boxes of the same
dyeing method, it does not
form a rectangle and it is
not possible to execute the
measurement.
.