Olympus FLUOVIEW FV1000 User Manual page 169

[Virtual Channels] group box
Show the virtual channels.
Click a channel checkbox to check it
makes the virtual channel available.
Select the dyeing method from the
drop-down list and select the group No.
in the [Group] drop-down list.
[Assign Filters and Laser by Dye]
check box
When checked, settings of filter and
laser will be automatically done
according to selection of
method
6. Select <SU Control> button of the [Settings] sub-panel in the [Acquire] panel.
In the [Optical System Configuration] window, select the conditions such as laser and
filter settings of each group.
For details on the [Optical System Configuration] window, see section 1-3-2-6, "Setting
the Filters".
(Example: Set the group No. to [2] in the [Group #] drop-down list of [For Seq. Scan].
Then open the [Group] drop-down list of vCh (2) in the [Virtual Channel]
group box and select [2].
In the [Dye] drop-down list of vCH (2), select [CY5].
In the [Excitation DM] drop-down list, select [DM488/543/633].
In the [Beam Splitter] drop-down list, select [Glass].
In the [Barrier Filter] drop-down list for CH2, select [BA660IF].
In the [Laser Unit] group box, click the picture of shutter and engage the
HeNe-R laser in the light path.
After completing the setup, select the <Close> button to close window.
Use this group box when performing sequential scan.
Select the group to be set in the [Group] drop-down list
and set the laser and filter settings in the [DMs & Filters in
SU] group box to change the settings of each group.
dye
APPLIED OPERATIONS
[For Seq. Scan] group box
/Image Acquisition
OPERATION INSTRUCTIONS
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