Olympus FLUOVIEW FV1000 User Manual page 106

APPLIED OPERATIONS
5 Setting the XY Observation Mode
6 Repeated Scanning Operation
<XY Repeat> button
<Focus> button
7 Setting the Multiple sections to be Observed
<Z stage fine adjustment> buttons
Displace the Z stage by 0.1 µm per
step.
OPERATION INSTRUCTIONS
Page
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/Image Acquisition
1. In the [Mode] group box in the [Acquire] panel, select the [Surface] option button.
2. In the [Acquire] panel, select the XY observation mode option button.
1. Select the <XY Repeat> button. The acquired image will be displayed in the [Live]
panel.
TIP
Use the <FOCUS> button to acquire image at an even higher speed. If the
specimen is already being scanned, stop scanning with the <STOP SCAN>
button before selecting the <XY Repeat> button.
The Focus function reduces the scanning time by line skipped scan. As a
result, the acquired images become coarse.
While acquiring image, move the Z stage to select the multiple sections to be observed.
From the panel page tabs shown on the bottom right of the [Acquire] panel, select the [Z
Stage] sub-panel.
Fig. 2-9 [Z Stage] Sub-panel
[Current Pos] text box
Shows the current position of the
stage. The value can also be
entered directly from the keyboard.
<Z stage coarse adjustment>
buttons
Displace the Z stage by 1.0 µm
per step.
[Locked] check box
Z-motor is engaged by checking this
box.