Other Observation Methods; Differential Interference Contrast Observation - Olympus BX51WI Instructions Manual

OTHER OBSERVATION METHODS

5-1 Differential Interference Contrast Observation

# The normal optical performance of DIC observation cannot be manifested if a plastic petri dish is used.
}DIC prisms (for revolving nosepiece and condenser), an analyzer and a polarizer are required for DIC observation.
When the reflected light fluorescence illuminator is not used, the U-KPA intermediate tube is required to attach the analyzer.
|
@
²
Fig. 28
@
³
Fig. 29
@
Fig. 30
1
Attaching the Analyzer
With the U-ANT Analyzer
}Drop the U-ANT analyzer in the dummy slider of the U-KPA intermediate
tube.
1. Place the U-ANT @ with the side with indications facing up, align the
³
indices and drop the analyzer into the dummy slider ² (the analyzer will
be absorbed by magnet).
2. Set the dummy slider ² back into the U-KPA ³ and tighten the clamp-
ing knob |.
With the U-AN Analyzer
}Insert the U-AN into the analyzer insertion slot of the reflected light fluores-
cence illuminator. (Refer also to the instruction manual of your reflected
light fluorescence illuminator.)
2
Attaching the Polarizer
}The performance of polarizer drops after it has been subjected to light for
long hours. Replace it after about 500 hours of continuous use.
Drop the polarizer into the filter insertion position @ or ² with a push ring,
and clamp with the push ring.
}It is recommended to insert the polarizer in insertion position @. This is
because the polarizer rotation clamping knob ³ comes on the front of
the microscope when the insertion position @ is engaged in the light
²
path.
}If you use the 32PO polarizer, the adjustment will be easier than with the
32POIR because the 32PO is brighter.
Also, the adjustment for IR reservation can be conducted under brighter
light if you remove the IR filter (32BP775 or 32IR900) during adjustment.
When Using the U-UCD8
# The polarizer built into the U-UCD8 is not necessary with this micro-
scope. Remove it as described below.
1. Using a Phillips precision screwdriver, remove the 6 clamping screws @
retaining the polarizer cover at the bottom side of the condenser.
2. Remove the cover to expose the polarizer and remove it together with
the frame. (Retain the removed polarizer carefully for future use with a
microscope other than the BX51WI.
3. Attach the polarizer cover to the original position.
BX51WI
(Fig. 28)
(Fig. 29)
19