Olympus FLUOVIEW FV1000 User Manual page 165

7. Among the laser <Off> buttons on the right of the [Laser Intensity] scales in the [Lasers]
group box, click the <Off> button of the laser you want to use in transmitted observation.
This should change the <Off> button to the <Rdy> button (displayed in the pressed-in
condition).
8. Click the <OK> button in the [FLUOVIEW] dialog box.
Image acquisition starts.
9. Adjust the brightness with the [PMT], [Offset] and [Gain] LED sliders in the [Ch] group
box in the [Acquire] panel. See section 2-2-1-4-9, "Adjusting the Image Brightness" for
details.
10. After acquiring images, select <Normal> from the list displayed below the [Surface XY-
Norm] option button in the [Mode] group box to set the scanning range to the original
setting.
APPLIED OPERATIONS
Fig. 2-25 [Lasers] Sub-panel
/Image Acquisition
<Off> button
Select the laser type.
OPERATION INSTRUCTIONS
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