Olympus FLUOVIEW FV1000 User Manual page 76

Getting Started FLUOVIEW/
OPERATION INSTRUCTIONS
Page
1 - 5 4
Online Help
The formulas of resolution of X and Y are as follows;
FWHM : Resolution of X and Y
L : The length of a diagonal line of Square Pinhole
NA = nSINθ :NA of Objective lens
MG : The objective Magnification
N : The refractive index of a medium
Air : 1.0
Water : 1.3
Oil : 1.5
X = L/(1.22*3.8*λ*MG/NA)
The case where the variable x is as follows; 0 <= x < 0.5
FWHM = (0.5λ*XYPSF)/NA
6
XYPSF = -5.1459x + 1.0147*10x
-1
7.1915*10
The case where the variable x is as follows; 0.5 <= x < 1.0
FWHM = (0.5λ*XYPSF)/NA
6
XYPSF = -3.05539x + 1.32031*10x
-1
+ 6.20850*10
The case where the variable x is as follows; 1.0 <= x < 1.7
FWHM = (0.5λ*XYPSF)/NA
6
XYPSF = -2.61786x + 2.14478*10x
6.17335*10x - 1.21495*10
The case where the variable x is as follows; 1.7 <= x < 2.0
FWHM = (0.5λ*XYPSF)/NA
3
XYPSF = -0.0078x + 0.0422x
The case where the variable x is as follows; 2.0 <= x
FWHM = (0.5λ*XYPSF)/NA
XYPSF = 1.0164
One Point!
5 4
- 7.1668x + 2.2804x
5 4
- 2.25074*10x + 1.89617*10x
5 4
- 7.18428*10x + 1.25767*10
2
- 0.0689x + 1.0478
3 -1 2
- 1.7696*10 x + 1.9256*10
3 2
- 7.89685x + 1.56587x
2 3
x - 1.21445*10
-02
x +
2 2
x +