Olympus FV1000 Manuallines
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Olympus FV1000 Manuallines

Confocal microscope
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Olympus FV1000 confocal microscope
Please
read and follow our
•
don´t use without introduction by
•
Only switch on parts you need for your purposes
•
(e.g. if you don't need the Hg lamp for epifluorescence like for overnight on confocal
microscopes)
report problems to
•

Laserlines:

Laser diode 405 nm (DAPI, Photoactivation, Uncaging)
Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)
DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)
Red Diode Laser: 635 nm (Cy5, Alexa 633/647)

Switching on the system:

Switch on the PC (button next to floppy drive).
•
Switch on the microscope components with the power manager (Mic-
•
control, PSU1+2, HBO lamp if neccessary)
Switch on the lasers:
•
A) Laser combiner (FV10-MCPSU)
B) 559 nm laser if needed
1) Main switch
2) Wait until 'TEMP' is not blinking anymore
3) Turn key to 'on' position
.
guidelines
us
without delay
ALMF
staff

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Summary of Contents for Olympus FV1000

  • Page 1 Olympus FV1000 confocal microscope Please read and follow our guidelines • don´t use without introduction by ALMF staff • Only switch on parts you need for your purposes • (e.g. if you don’t need the Hg lamp for epifluorescence like for overnight on confocal...
  • Page 2 C) Argon-laser if needed: 1) Main switch 2) Key switch NEW: Always turn off key switch, if the laser is not needed for more than 1 hour (e.g. next booking >1 hour later) !
  • Page 3 Log on to with the Username "ALMF". • Start the microscope control software using the icon on the desktop • Switching off Check if someone is booked after you on the same day! In case someone is • booked on the same day, just turn the intensity turnknob of the Argon-laser to the minimum position, switch off the key switch of the 559 nm laser, if there is at least 1 hour to next booking and leave everything else on.

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