Olympus Fluoview FV1000 Overview
Confocal laser scanning biological microscope
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Summary of Contents for Olympus Fluoview FV1000
- Page 1 Confocal Laser Scanning Biological Microscope FV1000 FLUOVIEW FLUOVIEW—Always Evolving...
- Page 2 The FV1000 also measures diffusion coefficients of intracellular molecules, quantifying molecular kinetics. Quite simply, the FLUOVIEW FV1000 represents a new plateau, bringing “imaging to analysis.” Olympus continues to drive forward the development of FLUOVIEW microscopes, using input from researchers to meet their evolving demands and bringing “imaging to analysis.”...
- Page 3 Imaging to Analysis ing up New Worlds From Imaging to Analysis FV1000 Advanced Deeper Imaging with High Resolution FV1000MPE...
- Page 4 Advanced FLUOVIEW Systems Enhance the Power of Your Research Superb Optical Systems Set the Standard for Accuracy and Sensitivity. Two types of detectors deliver enhanced accuracy and sensitivity, and are paired with a new objective with low chromatic aberration, to deliver even better precision for colocalization analysis. These optical advances boost the overall system capabilities and raise performance to a new level.
- Page 6 Excellent Precision, Sensitivity and Stability. FLUOVIEW Enables Precise, Bright Imaging with Minimum Phototox Main scanner Barrier filter Grating Grating Laser combiner LD635 Broadband fiber * Confocal pinhole LD559 AOTF LD473 AOTF LD405 Broadband fiber Laser combiner/Fiber Scanners/Detection Diode Laser Laser Combiner High Sensitivity Detection System Greater stability, longer service life and High-sensitivity and high S/N ratio optical...
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Page 7: Optical System
High S/N Ratio Objectives with Suppressed Autofluorescence Olympus offers a line of high numerical aperture objectives with improved fluorescence S/N ratio, including objectives with exceptional correction for chromatic aberration, oil- and water- immersion objectives, and total internal reflection fluorescence (TIRF) objectives. - Page 8 Two Versions of Light Detection System that Set New Standards for Optical Performance. Spectral Based Detection Flexibility and High Sensitivity Spectral detection using gratings for 2 nm wavelength resolution and image acquisition matched to fluorescence wavelength peaks. User adjustable bandwidth of emission spectrum for acquiring bright images with minimal cross- talk.
- Page 9 Technology / Hardware SIM Scanner Unit for Simultaneous Laser Light Stimulation and Imaging. SIM (Simultaneous) Scanner Unit Combines the main scanner with a dedicated laser light Lasers are used for both imaging stimulation scanner for investigating the trafficking of fluorescent- and laser light stimulation.
- Page 10 New Objective with Low Chromatic Aberration Delivers World-Leading Imaging Performance. Low Chromatic Aberration Objective Best Reliability for Colocalization Analysis A new high NA oil-immersion objective minimizes chromatic aberration in the 405–650 nm region for enhanced imaging performance and image resolution at 405 nm. Delivers a high degree of correction for both lateral and axial chromatic aberration, for acquisition of 2D and 3D images with excellent and reliable accuracy, and improved colocalization analysis.
- Page 11 Special Multiphoton Objective with Outstanding Brightness and Resolution Olympus offers a high NA water-immersion objective designed for a wide field of view, with improved transmittance at near- infrared wavelengths. A correction collar compensates for spherical aberration caused by differences between the refractive indices of water and specimens, forming the optimal focal spot even in deep areas, without loss of energy density.
- Page 12 User-Friendly Software to Support Your Research. Configurable Emission Wide Choice of Scanning Modes Image Acquisition by Application Wavelength Several available scanning modes User-friendly icons offer quick access to including ROI, point and high-speed functions, for image acquisition according Select the dye name to set the optimal bidirectional scanning.
- Page 13 Technology / Hardware Optional Software with Broad Functionality. Diffusion Measurement Package For analysis of intracellular molecular interactions, signal transduction and other processes, by determining standard diffusion coefficients. Supports a wide range of diffusion analysis using point FCS, RICS and FRAP. Multi Stimulation Software Configure multiple stimulation points and conditions for laser light stimulation...
- Page 14 Broad Application Support and Sophisticated Experiment Control. Multi-Color Imaging Measurement 3D/4D Light Stimulation Volume Rendering Multi- Dimensional Colocalization Time-Lapse FRET 3D Mosaic Imaging...
- Page 15 Application Measurement Diffusion measurement and molecular interaction analysis. Light Stimulation FRAP/FLIP/Photoactivation/Photoconversion/Uncaging. Multi-Dimensional Time-Lapse Long-term and multiple point. 3D Mosaic Imaging High resolution images stitched to cover a large area. Multi-Color Imaging Full range of laser wavelengths for imaging of diverse fluorescent dyes and proteins.
- Page 16 Diffusion Measurement Package This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular sizes and speeds.
- Page 17 Application/ Molecular Interaction Analysis RICS Application and Principles Comparison of Diffusion Coefficients for EGFP Fusion Proteins Near to Cell Membranes and In Cytoplasm RICS can be used to designate and analyze regions of interest At cytoplasmic membrane In cytoplasm based on acquired images. Diffusion coefficient D =0.98 µm Diffusion coefficient D =3.37 µm EGFP is fused at protein kinase C (PKC) for visualization, using...
- Page 18 Laser Light Stimulation The SIM scanner system combines the main scanner with a laser light stimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous imaging of the effects of stimulation on the cell.
- Page 19 Application/ Molecular Interaction Analysis Photoconversion The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed to light of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes from green to red. This phenomenon can be used to mark individual Kaede-expressing target cells among a group of cells, by exposing them to laser light.
- Page 20 Multi-Dimensional Time-Lapse The FV1000 can be used for ideal multi-dimensional time-lapse imaging during confocal observation, using multi-area time-lapse software to control the motorized XY stage and focus compensation. Significantly Improved Long Time-Lapse Throughput Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system efficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single experiment to increase the efficiency of experiments.
- Page 21 Application/ Molecular Interaction Analysis 3D Mosaic Imaging Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the images. The entire process from image acquisition to tiling can be fully automated. Mosaic Imaging for 3D XYZ Construction Composite images are quickly and easily prepared using the stitching function, to form an image over a wide area.
- Page 22 Calculate of Pearson coefficients, Equipment Required: PLAPON 60xOSC overlap coefficients and colocalization indices. * SIM scanner and TIRFM scanner cannot be installed on the same system. ** For more information about peripheral equipment, contact your Olympus dealer.
- Page 23 Expandability mCherry...
- Page 24 Scanning Units Two types of scanning units, filter-based and spectral detection, are provided. The design is all-in-one, integrating the scanning unit, tube lens and pupil projection lens. Use of the microscope fluorescence illuminator light path ensures that expandability of the microscope itself is not limited.
- Page 25 * Requires IX81 microscope. For information about ZDC- completely stable, at just below 37°C easy to perform with this motorized compatible objectives, contact your Olympus dealer. temperature, 90% moisture and 5% stage, which can reproduce previously- concentration; in this way, live cell set positions with extreme precision.
- Page 26 Image Format OIB/ OIF image format 8/ 16 bit gray scale/index color, 24/ 32/ 48 bit color, JPEG/ BMP/ TIFF/ AVI/ MOV image functions Olympus multi-tif format Spectral Unmixing 2 Fluorescence spectral unmixing modes (normal and blind mode) Image Processing...
- Page 27 Expandability Dimensions, Weight and Power Consumption Dimensions (mm) Weight (kg) Power consumption Microscope with scan unit BX61/BX61WI 320 (W) x 580 (D) x 565 (H) — IX81 350 (W) x 750 (D) x 640 (H) Fluorescence illumination unit Lamp 180 (W) x 320 (D) x 235 (H) Power supply 90 (W) x 270 (D) x 180 (H) AC 100-240 V 50/60 Hz 1.6 A...
- Page 28 FLUOVIEW website www.olympusfluoview.com • OLYMPUS CORPOARATION is ISO9001/ISO14001 certified. • Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. • Windows is a registered trademark of Microsoft Corporation in the United States and other countries.
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