Olympus FLUOVIEW FV1000 User Manual page 147

Confocal laser scanning biological microscope
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APPLIED OPERATIONS
/Image Acquisition
With types of dyeing of the specimen to observe, the barrier filter will be set
automatically to the optical path.
To change the barrier filter types, see section 1-3-2-3, "Configuring the Filters (When
using a filter system)" and change by [Optical System Configuration] window.
Make the channel ready for image acquisition.
In the [Acquire] panel, make sure that the check box in the [Ch1]/[Ch2]/[Ch3]/[Ch4]
group box that shows the dyeing method is check-marked to indicate that the
corresponding channel is ready for image acquisition.
Ch4 check box
Ch3 check box
Ch2 check box
Ch1 check box
Set the image brightness.
Adjust the image brightness of the acquisition channel by using the [PMT], [Offset] and
[Gain] LED sliders in the [Acquire] panel. For details, see to section 2-2-1-4-9,
"Adjusting the Image Brightness" in the [OPERATION] volume.
OPERATION INSTRUCTIONS
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