Repeatability Test With Uv-Detection - Thermo Scientific Accela Open Autosampler Hardware Manual

6
Troubleshooting
Standard Chromatographic Tests

Repeatability Test with UV-Detection

98 Accela Open Autosampler Hardware Manual
Table 32. Carryover tests with UV-detection (Sheet 2 of 2)
Parameter
Needle Gap Valve Clean
Fill Speed
Fill Strokes
Stator Wash
Pullup Del
Inject to
Inject Speed
Pre Inj Del
Pst Inj Del
Valve Clean Time Solvent 2
Post Clean Time Solvent 2
Valve Clean Time Solvent 1
Post Clean Time solvent 1
Table 33 lists the specifications for repeatability tests with UV-detection.
Table 33. Repeatability tests with UV-detection (Sheet 1 of 2)
Component
Test Sample
HPLC System
Wash Solvents
Value
3 mm
5 μL/s
0
0
500 ms
Specified Inj. Valve
5 μL/s
500 ms
500 ms
2 s
2 s
3 s
2 s
Specification
• Loop overfill: Caffeine, 520 mg/L
• Partial loop: Caffeine, 52 mg/L dissolved in
water:acetonitrile = 90:10 + 0.1%TFA
• Binary solvent delivery pump
• UV-Detector, wavelength: 273 nm
• HPLC column: Hypersil Gold C18, 2.1 × 50 mm
spherical 1.9 μm
• Column temperature: Ambient
• Mobile phase
Water:acetonitrile = 90:10 +0.1% TFA
• Gradient: Isocratic for 2 min
• Wash1: Water + 0.1% TFA
• Wash2: Acetonitrile + 0.1% TFA
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