Standard Chromatographic Tests - Thermo Scientific Accela Open Autosampler Hardware Manual

6
Troubleshooting

Standard Chromatographic Tests

Standard Chromatographic Tests
96 Accela Open Autosampler Hardware Manual
Expected result: Collection of approximately 8 mL
(Water, DLW syringe needle, gauge 22)
 (Test 2) To test the pump flow delivered at the pump outlet
1. Disconnect the outlet tube from the pump module.
2. Connect PEEK tubing with the following dimension to the outlet of the pump module:
85 mm (3.35 in.) ID, 0.25 mm (0.010 in.) ID.
3. If necessary, replace the solvent in the reservoir bottle with water.
4. Activate the pump by selecting this path:
Menu > F3-Setup > Objects > Events > Pwr-Out1 or Pwr-Out2
5. Collect the pumped water in a graduated cylinder.
Expected Result: Within 30 seconds, pump delivery of approximately 10 mL
This short test can indicate whether the system is performing as expected. If you observe no or
very little flow, systematically check using a process of elimination. For more details, see
Table 29.
This section describes standard chromatographic tests for carryover and repeatability. These
tests provide basic information for both users and technical support.
The tests described are examples only; vary them accordingly if the configuration of the
HPLC system changes.
The first test uses PAL standard valves with a bore size of 0.25 mm to provide a flow rate
range of 10–500 μL/min.
Both tests, carryover and repeatability, test conditions for UV and LC/MS detection.
Details are listed in the next section. Note that the given levels to reach for carryover and
repeatability do not reflect the specification value. These described tests should give you
confirmation if the system is working to expectation using reasonable effort and time. The
tests are more comparable with an OQ Test (Operational Qualification Test).
Note The levels provided in these tests do not reflect the specification value for careover
or repeatability. The test results will confirm if the system is working properly with
reasonable effort and within the expected time frame. The tests are comparable to
Operational Qualification (OQ) tests.
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