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ProFlex
PCR System Installation and Operation
Catalog Numbers 4483636, 4483637, 4483638, 4484071, 4484073, 4484074, 4484075, 4484076, 4484078
Pub. No. 4483807 Rev. G
Note: For safety and biohazard guidelines, see the "Safety"
appendix in the ProFlex
PCR System User Guide (Pub. No.
MAN0007697). Read the Safety Data Sheets (SDSs) and follow
the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves.
Product description
This document summarizes procedures for installing and using
the ProFlex
Thermal Cycler, with "better than gradient
technology. For detailed instructions, see the ProFlex
System User Guide (Pub. No. MAN0007697).
Figure 1 ProFlex
3 × 32‑Well PCR System
Required materials not provided
• Can of compressed air (For use in cleaning wells of sample
block).
• (Optional) Electrical protective devices.
Note: The use of one or more of the following electrical
protective devices is recommended.
·
Power line regulator (1.5 kVA)
·
Surge protector/line conditioner (10-kVA)
·
Uninterruptible power supply (1.5-kVA)
For Research Use Only. Not for use in diagnostic procedures.
Set up the ProFlex
IMPORTANT!
need to ship the instrument in for service.
1. Open the shipping crate to unpack the ProFlex
Cycler. The instrument is contained in three boxes; one with
the base module, a second with the sample block, and the
third containing accessories.
" VeriFlex
2. Remove the packaging material, then inspect the instrument
PCR
for shipping damage.
Figure 2 ProFlex
Figure 3 ProFlex
accessories packaging
3. Use compressed air to clear out each well of the sample
block to remove particles that may have collected inside
during shipping.
4. Place the instrument at an installation site that:
QUICK REFERENCE
Thermal Cycler
Save the packing materials and box in case you
Base module packaging
3x32-Well Sample Block and
Meets the spatial and weight requirements for the
thermal cycler (see "Technical specifications" on
page 7).
Is within 1 m (3 ft) of a 800-VA power receptacle.
Thermal

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Summary of Contents for Thermo Scientific Applied Biosystems ProFlex PCR System

  • Page 1 QUICK REFERENCE ProFlex PCR System Installation and Operation ™ Catalog Numbers 4483636, 4483637, 4483638, 4484071, 4484073, 4484074, 4484075, 4484076, 4484078 Pub. No. 4483807 Rev. G Set up the ProFlex Thermal Cycler Note: For safety and biohazard guidelines, see the “Safety” ™...
  • Page 2 5. Turn the base module to view the rear of the instrument. Insert the Wi-Fi Module into the USB port at the back of the instrument. For instructions on setting up the wireless connection, see “How to set up the wireless connection for the ProFlex Thermal Cycler”...
  • Page 3 1. Press (Sign In) > Sign In, then select the Administrator How to set up the wireless connection for the profile. ProFlex Thermal Cycler ™ 2. Log in. (The default password for the Administrator profile is See “Set up the ProFlex Thermal Cycler”...
  • Page 4 ™ Note: For the ProFlex 3x32 Well PCR System, you will need Connect by mobile device to choose a block prior to starting a run. 1. Download the "Instrument Connect Mobile Application" on your mobile device. Maintenance guidelines ™ ™ a.
  • Page 5 Prepare samples using MicroAmp Reaction Tubes or Prepare samples using MicroAmp tubes/tube strips ™ ™ Tube Strips with separate cap strips 1. Pipette the reaction mixture into the reaction tubes. 1. Separate the blue tray and retainer by squeezing the release catch as indicated in the graphic.
  • Page 6 Prepare samples using MicroAmp tube strips with Seal tubes strips with cap strips ™ attached caps IMPORTANT! Apply significant downward pressure on the sealing tool in all steps to form a complete seal on top of the 1. Separate the blue tray and retainer by squeezing the release tubes.
  • Page 7: Technical Specifications

    Technical specifications ™ ™ The following table displays the system specifications for an instrument using the ProFlex 96-Well Sample Block, ProFlex 3x 32-Well ™ ™ ™ Sample Block, ProFlex 2x 96-Well Sample Block, ProFlex 2x Flat Sample Block, ProFlex 2x 384-Well Sample Block Table 1 ProFlex Thermal Cycler technical specifications: ProFlex...
  • Page 8: Accessory Products

    Accessory products Product Cat. No. 96-well 0.2-mL reaction plates MicroAmp Optical 96-Well Reaction Plate N8010560, 4316813 ™ MicroAmp Optical 96‑Well Reaction Plate with Barcode 4306737, 4326659 ™ MicroAmp Optical 96-Well Reaction Plate with Barcode & Optical Caps 403012 ™ MicroAmp Optical 96-Well Reaction Plate with Barcode &...
  • Page 9: Limited Product Warranty

    Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at www.thermofisher.com/support. Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.