Thermo Scientific Accela Open Autosampler Hardware Manual page 49

Thermo Scientific
 To connect the DLW tubing to the syringe holder
1. Connect the end of the sleeved tubing with the short tubes to the DLW Syringe Holder
assembly. See "Mounting the DLW Pump Holder "
2. Use the groove in the block at the back of the DLW Manifold to orient the guide wire. To
connect, use the provided screw (M3×5) and the corresponding serrated lock washer.
3. Test to see if the wire tension is enough to keep the tubing in an upright position but low
enough to move the syringe holder manually along the x axis.
IMPORTANT The design of the DLW Manifold and the concept of washing by active
pumping and active closing of the lines by the DLW Actuator theoretically do not
require a predefined solvent line position.
A problem could occur if solvent is refilled or exchanged while the tubing still
contains solvent from the previous setup. Always keep the lines at the same position,
and prime the entire system with caution to prevent unnecessary confusion.
4. Connect solvent line 1 to the upper port and solvent line 2 to the lower port at the left
side of the DLW Manifold.
Make sure the order of the tube connections, upper or lower, is consistent. In certain
applications you cannot mix types of solvents; for example, biofluid sample solution
should not come in contact with highly concentrated organic solvents.
Inserting the DLW Syringe Holder Assembly into the Injection Unit
Tip Insert the DLW Syringe Holder assembly when the Open Autosampler is powered off.
 To insert the DLW Syringe Holder assembly into the injection unit
1. Manually move the PAL injection unit aside to allow free movement of the syringe slider.
Lower the syringe slider
assembly.
Match the magnetic pins of the syringe holder using the counter positions at the syringe
slider.
(Figure 25) in order to gain access to install the DLW Syringe
Accela Open Autosampler Hardware Manual
2
Installing the Accela Open Autosampler
Installing the Autosampler and DLW
on page 23.
31