Thermo Scientific Accela Open Autosampler Hardware Manual page 106

6
Troubleshooting
Troubleshooting Using Observed Symptoms or Error Messages
Table 29. Troubleshooting using symptoms and error messages (Sheet 4 of 5)
Symptom or error message
Sample peaks/responses not
reproducible
Excessive carryover between
samples
88 Accela Open Autosampler Hardware Manual
Possible cause
Dirty syringe or Holding Loop
Syringe pressure differences
Vacuum created in sample vial
Method parameters
Loose, unstable, or wrong
connection
Inappropriate wash solvent or
solvents
Waste tubing ID too small at
injection valve, causing waste liquid
to be pulled back, by capillary
action, into rotor groove
Air gaps not formed. Liquids of
different type blending into each
other.
Recommended action
Increase Pst Cln Slv1/Slv2 values in method. Use
PreClnSlv1/Slv2 and PreClnSpl.
Increase Pullup Delay value.
Reduce sample volume in sample vial. Use
setting under F3-Setup > System >
PrePressureVial.
Check recommended Method parameters in
"PAL Firmware Overview"
verify these critical parameters:
• Fill speed
• Pullup delay
• Injection Speed
• Post Inj Delay
Check all connections within the DLW system,
such as needle, Holding Loop, injection loop
connections, and so on.
Use appropriate wash solvents.
Replace the waste tubing with larger ID tubing.
Verify the DLW method parameter for Airgap
Volume.
Prime entire system.
on page 136. First
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