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Summary of Contents for Thermo Scientific Accela
- Page 1 Accela RI Detector Hardware Manual 60257-97002 Revision A February 2010...
- Page 2 © 2010 Thermo Fisher Scientific Inc. All rights reserved. ChromQuest and Accela are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Luer-Lok is a registered trademark of Becton, Dickinson and Company in the United States. Teflon is a registered trademark of E. I. du Pont de Nemours and Company in the United States and other countries.
- Page 3 Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards, replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives. Accela RI Detector EMC Directive 2004/108/EC EMC compliance has been evaluated by TÜV Rheinland of North America, Inc.
- Page 4 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument.
- Page 5 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
- Page 6 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
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Page 9: Table Of Contents
Connecting the Inlet and Outlet Tubing......18 Turning On the Accela RI Detector for the First Time ....21 Chapter 3 Routine Maintenance . - Page 10 Index ..............37 Accela RI Detector Hardware Manual...
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Page 11: Preface
RI Detector. Related Documentation In addition to this guide, the Thermo Scientific data system that you use to control the Accela RI Detector has a built-in Help system. Thermo Fisher Scientific provides the following documents as PDF files for the Accela RI Detector: •... -
Page 12: Contacting Us
To suggest changes to documentation or to Help • Complete a brief survey about this document by clicking the link below. Thank you in advance for your help. • Send an e-mail message to the Technical Publications Editor at techpubs-lcms@thermofisher.com. Accela RI Detector Hardware Manual Thermo Scientific... -
Page 13: Introduction
Introduction The Accela RI Detector (Figure 1) is a member of the Accela family of LC instruments. Contents • Functional Description • Front and Back Panels • Status LEDs • Specifications Figure 1. Accela RI Detector Thermo Scientific Accela RI Detector Hardware Manual... -
Page 14: Functional Description
4 Optical System The optical design of the Accela RI Detector is based on Snell’s Law of Refraction, which uses a prism flowcell. The detector has a flowcell with two compartments—sample and reference—that form a pair of liquid prisms. - Page 15 When the refractive indices of the sample and reference solvents in the flowcell differ, the image on the photodiode moves horizontally as illustrated in Figure Thermo Scientific Accela RI Detector Hardware Manual...
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Page 16: Flowcell
• When the Purge Control is On, solvent flows through both the sample and the reference compartments of the flowcell. • When the Purge Control is Off, the reference compartment of the flowcell is shut off from the solvent path. Accela RI Detector Hardware Manual Thermo Scientific... - Page 17 316 stainless tubing NC = Normally closed NO = Normally open 316 stainless tubing (closed when purge control is off) COM = Valve exit Teflon™ tubing Connection by female nut Connection by flange Thermo Scientific Accela RI Detector Hardware Manual...
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Page 18: Front And Back Panels
Introduction Front and Back Panels Front and Back Panels Figure 6 Figure 7 show the front and back panels of the Accela RI Detector. Figure 6. Front panel LEDs CCELA RI Detector Power Comm Temp Power switch Figure 7. Back panel... -
Page 19: Status Leds
Introduction Status LEDs Status LEDs The Accela RI Detector has four status light-emitting diodes (LEDs) located on the left door of the detector as shown in Figure 8 and described in Table Figure 8. Status LEDs Power Comm Temp Table 1. Status LEDs and meanings... - Page 20 Introduction Specifications Table 2. Accela RI Detector specifications (Sheet 2 of 2) Item Specification Fuse size • For 110/115 V ac: T3.15 A, 5×20 mm • For 230 V ac: T1.6A, 5×20 mm Operating humidity 5% to 95% non-condensing relative humidity...
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Page 21: Installation
Installation This chapter describes the initial installation of your Accela RI Detector, including how to connect it to the other modules of the Accela LC system. Contents • Installation Checklist • Unpacking and Inspecting • Checking the Fuses • Loosening the Transport Screws •... -
Page 22: Installation Checklist
“Accessory Kit” page If any items are missing, contact your Thermo Fisher Scientific representative immediately. Note Before you install the software driver for the Accela RI Detector you must first install the ChromQuest chromatography data system. Accela RI Detector Hardware Manual... -
Page 23: Checking The Fuses
Installation Checking the Fuses Checking the Fuses You can operate the Accela RI Detector at 100/115V or 230V. However, ensure that the appropriate fuses are installed (Figure 9 Table CAUTION Do not connect the power cord to your instrument without first verifying that... -
Page 24: Loosening The Transport Screws
Loosening the Transport Screws To prevent damage that might occur during shipment, the optical block of the Accela RI Detector is held in place by two transport screws (5 mm bolts) located underneath the detector along the right side. To use the detector, loosen the transport screws with a 5 mm hexagonal wrench, provided in the accessory kit. -
Page 25: Placement And Environmental Requirements
• A stable room temperature (± 0.5 °C) for applications requiring maximum detection sensitivity. • Clearance of at least 15 cm (6 in.) between the back panel of the Accela RI Detector and any wall or obstruction. This clear space provides access to the back-panel connectors and a free flow of cooling air. -
Page 26: Making The Back Panel Connections
Making the Back Panel Connections Figure 12. Accela LC system Making the Back Panel Connections Use the cables provided in the Accela RI Detector accessory kit to make the back panel connections described in this section. This section contains the following topics: •... -
Page 27: Connecting To The Data System Computer
2. Plug the Ethernet switch into the data system computer using the supplied CAT5, 7 ft long, shielded Ethernet cable with ferrite clamp. Note A CAT5 Ethernet cable is the only cable shipped with the Accela RI Detector. If you already have an Accela LC system, the Ethernet switch and system interconnect cable are already part of your existing system. -
Page 28: Connecting The System Interconnect Cable
To connect the Accela modules to the system interconnect cable 1. To connect the Accela RI Detector, plug one of the three connectors labeled DETECTOR to the left, 8-pin receptacle located on the back panel of the Accela RI Detector as shown in... - Page 29 9 10 11 12 13 14 15 16 UNIT ID ENET Accela autosampler Connector labeled PUMP DO NOT USE TOGETHER WITH D-SUB25 RS232 1 2 3 4 5 6 7 8 Accela pump Thermo Scientific Accela RI Detector Hardware Manual...
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Page 30: Connecting The Inlet And Outlet Tubing
Connecting the Inlet and Outlet Tubing To connect the inlet and outlet tubing to the detector 1. Remove the plugs from the IN and OUT ports on the front panel of the Accela RI Detector. Keep the plugs. 2. Connect one end of the 0.005 in. ID × 1/16 in. OD stainless steel inlet tubing supplied... - Page 31 (connection from Accela LC column) RI Detector Out port (connection to waste) Accela 3/32 in. OD × 0.060 in. ID autosampler Teflon outlet tubing Drain manifold Accela pump Convoluted tubing To waste container Thermo Scientific Accela RI Detector Hardware Manual...
- Page 32 Accela autosampler 0.005 in. ID × 1/16 in. OD stainless steel inlet tubing LC Column Accela RI Detector Accela pump 3/32 in. OD × 0.060 in. ID Teflon outlet tubing To waste container Accela RI Detector Hardware Manual Thermo Scientific...
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Page 33: Turning On The Accela Ri Detector For The First Time
(Figure 6 page 2. Ensure that the Accela RI Detector is properly connected to the data system computer. 3. Ensure that the data system lists the appropriate configuration settings for the Accela RI Detector. 4. Plug the power cord into the power receptacle on the back panel of the detector... -
Page 35: Routine Maintenance
Routine Maintenance This chapter describes how to maintain the Accela RI Detector. Proper maintenance ensures the optimum performance of the Accela RI Detector. You are responsible for maintaining your detector by properly performing the maintenance procedures on a regular basis. If you have any questions about proper maintenance, or to arrange for a preventive maintenance program, contact your Thermo Fisher Scientific field service engineer. -
Page 36: Preventive Maintenance
• To avoid particulate contamination and fluid path blockage, maximize the use of filtration. • Avoid leaving buffers standing in the flowcell for long periods. Plan your use to minimize standing time between runs. Accela RI Detector Hardware Manual Thermo Scientific... -
Page 37: Cleaning The External Surfaces Of The Detector
To flush the flowcell 1. Prepare a 20% (v/v) solution of nitric acid in HPLC-grade water. 2. Connect the LC pump directly to the Accela RI Detector. 3. Launch the ChromQuest data system. The Instrument window appears. 4. Choose Control > Instrument Status. -
Page 38: Replacing The Fuses
CAUTION To avoid an electrical shock, before you replace the fuses, turn off the power to the Accela RI Detector and unplug it from line power. 2. Squeeze the lever located just below the fuse holder cover until you hear a clicking sound,... - Page 39 4. Push the fuse holder back into the back panel of the detector until you hear a clicking sound. 5. Plug the power cord into the power receptacle on the back panel of the detector. 6. Turn the power on by pushing in the power switch on the front panel. Thermo Scientific Accela RI Detector Hardware Manual...
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Page 41: Troubleshooting
Troubleshooting This chapter contains helpful information for troubleshooting possible Accela RI Detector and chromatographic system problems. Because many of the problems attributed to the detector might actually be due to other components in the chromatographic system, references and potential solutions to these types of problems are also included. Some common error messages are also included. - Page 42 “Flushing the Flowcell” page Integrator input and output voltage Verify that the integrator is connected to the not matching appropriate analog output connections on the detector. Check the attenuation setting on the integrator. Accela RI Detector Hardware Manual Thermo Scientific...
- Page 43 “Specifications” page Power cord not connected Connect the power cord. Detector not running Detector not receiving Start signal Check the connection to the Run contacts on the upon injection back panel. Thermo Scientific Accela RI Detector Hardware Manual...
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Page 44: Error Messages
System Is Ready For Run but failed. Shut down and restart the instruments. Initialize Device For Run failed. Shut down and restart the instruments. Lost connection to Accela RI on stack. Check the instrument connections. Shut down and restart the instruments. Acquisition Is Complete but failed. - Page 45 Instrument connection loss Check Ethernet connection and start the software. Check the module configuration in the software. Extend Run failed Do not extend the run when the detector is not in run mode. Thermo Scientific Accela RI Detector Hardware Manual...
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Page 47: Appendix A Accessories And Maintenance Parts
Accessories and Maintenance Parts This appendix contains the lists of accessories and maintenance parts for the Accela RI Detector that you can order from Thermo Fisher Scientific. Contents • Accessory Kit • Fuses • Service Spare Parts Accessory Kit The accessory kit, listed in... -
Page 48: Fuses
Accessories and Maintenance Parts Fuses Note A CAT5 Ethernet cable is the only cable shipped with the Accela RI Detector. If you already have an Accela LC system, the Ethernet switch and system interconnect cable will be part of your existing system. If you just purchased an Accela LC system, locate the Ethernet switch and system interconnect cable in Accela system accessory kit. -
Page 49: Index
FCC compliance flowcell regulatory compliance bubbles in replaceable parts cleaning rise time description leaking schematic fuse size, checking safety standards fuses service spare parts replacing solvent lines, connecting specification specifications status LEDs storage temperature Thermo Scientific Accela RI Detector Hardware Manual... - Page 50 Index: T system interconnect cable temperature fluctuations, results of transport screws, loosening troubleshooting WEEE compliance weight Accela RI Detector Hardware Manual Thermo Scientific...
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