Carryover Test With Uv-Detection - Thermo Scientific Accela Open Autosampler Hardware Manual

Carryover Test with UV-Detection

Thermo Scientific
Table 31 lists the specifications for carryover tests with UV-detection.
Table 31. Specifications for carryover tests with UV-detection
Component
Test Sample
Blank Solution
HPLC System
Wash Solvents
Loop Size
Injection Valve
Cycle Composer Macro
or Cycle (ICC-CE)
Cycle Composer Method Parameter Settings
Table 32 lists the Cycle Composer Method parameter settings for carryover tests with
UV-detection.
Table 32. Carryover tests with UV-detection (Sheet 1 of 2)
Parameter
Syringe
Sample Volume
Airgap Volume
Front Volume
Rear Volume
Specification
Chlorhexidine, 0.6 mg/mL dissolved in
Water:acetonitrile = 90:10 + 0.1%TFA
Water:acetonitrile + 0.1%TFA
• Binary solvent delivery pump
• UV-Detector, wavelength: 257 nm
• HPLC column: C18 (Halo), 2.1 x 50 mm
spherical 2.7 μm
• Column temperature: Ambient
• Mobile Phase:
A: Water + 0.1%TFA
B: Acetonitrile + 0.1% TFA
• Gradient:
10% A hold for 0.5 min, to 90% B in 7.5 min
hold for 1 min
• Wash1: Water + 0.1% TFA
• Wash2: Acetonitrile + 0.1% TFA
2 μL; Loop ID 0.13 mm
VICI/Valco (CH-106678),
bore size 0.25 mm
DLW Standard
Value
100 μL DLW
7 μL
3 μL
5 μL
5 μL
6
Standard Chromatographic Tests
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