Thermo Scientific Dionex Chemically Regenerated Suppressor 500 Series Product Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Laboratory Equipment
  5. 500
  6. Product manual
Thermo Scientific Dionex Chemically Regenerated Suppressor 500 Series Product Manual

Thermo Scientific Dionex Chemically Regenerated Suppressor 500 Series Product Manual

Hide thumbs Also See for Dionex Chemically Regenerated Suppressor 500 Series:
Table of Contents

Advertisement

Quick Links

Thermo Scientific
Dionex CRS 500
Product Manual
P/N: 031727-07
March 2015
Part of Thermo Fisher Scientific

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Dionex Chemically Regenerated Suppressor 500 Series and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Thermo Scientific Dionex Chemically Regenerated Suppressor 500 Series

Summary of Contents for Thermo Scientific Dionex Chemically Regenerated Suppressor 500 Series

  • Page 1 Thermo Scientific Dionex CRS 500 Product Manual P/N: 031727-07 March 2015 Part of Thermo Fisher Scientific...
  • Page 2 Product Manual Dionex Chemically Regenerated Suppressor 500 Dionex ACRS 500, 4 mm, (P/N 085090) Dionex CCRS 500, 4 mm, (P/N 085092) Dionex ACRS 500, 2 mm, (P/N 085091) Dionex CCRS 500, 2 mm, (P/N 085093) Thermo Scientific Product Manual for Dionex CRS 500 Suppressor Page 2 of 40 031727-07...
  • Page 3 © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation.
  • Page 4 Safety and Special Notices Make sure you follow the precautionary statements presented in this guide. The safety and other special notices appear in boxes. Safety and special notices include the following: Indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

  • Page 5: Table Of Contents

    Contents Contents 1. Introduction ......................7 Chemically Regenerated Suppressor ....................7 Dionex Chemically Regenerated Suppressor Assembly Schematic ..........9 Chemical Suppression Schematic ....................11 Shipment and Storage ........................12 1.4.1 Shipment ............................. 12 1.4.2 Storage ..............................12 Overview of Suppression Modes ....................13 Mode of Operation Selection ......................
  • Page 6 Contents 4. Troubleshooting Guide ..................34 Small Analyte Peak Areas ......................34 High Background Conductivity ....................34 Drifting Baseline........................... 35 Decreased Sensitivity ........................36 System Back Pressure Increases Over Time ................36 Liquid Leaks ..........................37 5. Suppressor Cleanup ..................38 Base-Soluble Contaminants or Precipitates with the Dionex CRS 500 ........39 Organic Contaminants with the Dionex ACRS 500 or Dionex CCRS 500 ........

  • Page 7: Introduction

    – Introduction 1. Introduction Suppressor: The role of a suppressor in Ion Chromatography is to remove the eluent and sample counterions and replace this with regenerant ions thereby converting the eluent to a weakly dissociated form prior to detection. Detection of analyte ions particularly with conductivity detection is therefore feasible against a low background.
  • Page 8 – Introduction Figure 1 The Chemical Suppression System Eluent Out To Detector Cell with Back Pressure Coils and then to Waste to DCR Reservoir Eluent In From Analytical Column Regenerant is pumped in Regenerant is Diverted to Waste Recycled to AutoRegen Module The Dionex Chemically Regenerated Suppressors, Dionex Anion Chemically Regenerated Suppressor (Dionex ACRS 500) and the Dionex Cation Chemically Regenerated Suppressor (Dionex CCRS 500), provide a high performance, highly reliable, low maintenance chemical...

  • Page 9: Dionex Chemically Regenerated Suppressor Assembly Schematic

    – Introduction For assistance, contact Technical Support for Dionex Products. In the U.S., call 1-800- 346-6390. Outside the U.S., call the nearest Thermo Fisher Scientific office. NOTE 1.2 Dionex Chemically Regenerated Suppressor Assembly Schematic The Dionex CRS 500 includes two regenerant compartments and one eluent compartment separated by ion exchange membranes.
  • Page 10 – Introduction Figure 2 Membrane and Flow Chamber Configuration in the Dionex Chemically Regenerated Suppressors Regen Out Eluent In Regen In Eluent Out Thermo Scientific Product Manual for Dionex CRS 500 Suppressor Page 10 of 40 031727-07...

  • Page 11: Chemical Suppression Schematic

    – Introduction 1.3 Chemical Suppression Schematic Chemical Suppression with the Dionex ACRS 500 is a neutralization reaction and selective desalting process carried out across a pair of cation exchange membranes. The cation exchange membranes facilitate the selective exchange of cations in the suppressor. With sodium hydroxide as the eluent, sodium ions are continuously exchanged for hydronium ions from the regenerant acid and combine with the eluent hydroxide to form water.

  • Page 12: Shipment And Storage

    – Introduction 1.4 Shipment and Storage 1.4.1 Shipment The Dionex Chemically Regenerated Suppressors (Dionex ACRS 500 and Dionex CCRS 500) should not be subjected to temperatures above 60°C during shipment, storage or operation for extended periods of time. CAUTION 1.4.2 Storage Ensure the suppressor is stored in a temperature controlled environment away from direct exposure to sunlight or other sources of heat.

  • Page 13: Overview Of Suppression Modes

    – Introduction 1.5 Overview of Suppression Modes Three basic modes of suppression can be used with the Dionex Chemically Regenerated Suppressor (Dionex CRS 500): Displacement Chemical Regeneration Mode: This mode is a self-contained means of dispensing regenerant into the Dionex CRS 500 without any added pumps or delivery systems.

  • Page 14: Mode Of Operation Selection

    – Introduction 1.6 Mode of Operation Selection The Dionex ACRS 500 uses sulfuric acid as the preferred regenerant to achieve eluent suppression. The Dionex CCRS 500 uses tetrabutylammonium hydroxide (TBAOH) as the preferred regenerant to achieve eluent suppression. Both the Dionex ACRS 500 and Dionex CCRS 500 mode of operation is highly dependent on the ionic strength or concentration of the eluent used and the analysis sensitivity requirements.

  • Page 15: The Displacement Chemical Regeneration (Dcr) Mode

    – Introduction 1.6.1 The Displacement Chemical Regeneration (DCR) Mode The DCR Mode is the easiest mode to implement and provides convenient and economical operation in which the regenerant is displaced from the regenerant reservoir using the conductivity cell effluent. This mode delivers regenerant to the suppressor at a flow rate equal to the eluent flow rate.

  • Page 16: The External Chemical Mode

    – Introduction 1.6.2 The External Chemical Mode The External Chemical Mode is an easy to implement configuration that dispenses regenerant using a pressurized regenerant reservoir directly into the suppressor. The concentration of the regenerant dictates the leakage into the eluent stream therefore lower concentration of the regenerant is preferred in this mode.

  • Page 17: The Autoregen Suppression Mode

    – Introduction 1.6.3 The AutoRegen Suppression Mode This mode has been discontinued by Thermo Scientific and is only included here for legacy operators. The AutoRegen Suppression Mode is another mode for implementing chemical suppression. This mode is designed to recycle the regenerant and reduce regenerant consumption for high suppression applications.

  • Page 18: Installation

    – Installation Installation 2.1 System Requirements The Dionex Anion or Cation Chemically Regenerated Suppressor (Dionex CRS 500) is designed to be run on any Dionex Ion Chromatograph (IC) equipped with an ion exchange column set and suppressed conductivity detection. The Dionex CRS 500 is installed immediately after the analytical column and before the conductivity detector cell.

  • Page 19: Back Pressure Coils For The Dionex Crs 500

    – Installation During the course of installing and using the Dionex CRS 500, it may be necessary to assemble 1/4-28 or 10-32 ferrule/bolt style liquid lines. See, “Dionex Liquid Line Fittings,” for complete details. NOTE 2.2 Back Pressure Coils for the Dionex CRS 500 All detector cells require enough back pressure to prevent eluent in the cell from out-gassing due to abrupt volume changes between the small inner diameter of the connecting tube and the relatively larger volume of the cell.
  • Page 20 – Installation Table 2 Coils for Dionex CRS 500 Back Pressure Requirements Dionex Flow Rate I.D. of Appropriate Number CRS III Tubing Length of of Coils Type Each Coil 4 mm 0.5–1.5 ml/min 0.010" (Black) 2.5 feet 4 mm 1.5–3.0 ml/min 0.010"...

  • Page 21: Plumbing For Displacement Chemical Regeneration (Dcr) Mode Of Operation

    – Installation 2.3 Plumbing for Displacement Chemical Regeneration (DCR) Mode of Operation Figure 7 The Displacement Chemical Regeneration (DCR) Mode Plumbing Diagram Injection Valve Guard Column Waste Cell Analytical Regen Column Eluent Eluent Regen Regenerant The Displacement Chemical Regeneration Mode is the easiest method of operation. As the eluent passes through the suppressor, it is neutralized to produce its weakly ionized form.
  • Page 22 – Installation A. Install the Dionex Chemically Regenerated Suppressor (Dionex CRS 500) in the Chromatography Module. B. Connect the outlet of the analytical column to the ELUENT IN of the Dionex CRS 500. See Figure 7, “The Displacement Chemical Regeneration (DCR) Mode Plumbing Diagram.”...

  • Page 23: Plumbing For External Chemical Mode Of Operation

    – Installation 2.4 Plumbing for External Chemical Mode of Operation Figure 8 Configuration of the External Chemical Mode with the Dionex CRS 500 Suppressor Injection Valve Gas Pressure Coil #2 25 psi max Guard Column Coil #1 Waste Analytical Cell Regen Column Eluent...

  • Page 24: Peristaltic Pump Installation

    – Installation B. Make the following liquid line connections. See Figure 8, “Configuration of the External Chemical Mode with the Dionex CRS 500 Suppressor.” Use a coupler (P/N 039056) to connect one end of the 30" tubing assembly (P/N 035727) that comes in the Installation Kit to the water reservoir. Connect the other end of this tubing to the REGEN IN port of the Dionex Chemically Regenerated Suppressor.

  • Page 25: Plumbing For Autoregen Suppression Mode Of Operation

    – Installation 2.6 Plumbing for AutoRegen Suppression Mode of Operation Figure 9 Configuration of the Dionex CRS 500 Suppressor Using AutoRegen Injection Valve Coil #2 Guard Column Coil #1 Waste Analytical Cell Column Eluent Eluent Regen Regen Pressure Relief Assembly Regenerant Cartridge Pump...
  • Page 26 – Installation This mode has been discontinued by Thermo Scientific and is only included here for legacy operators. The AutoRegen Suppression Mode requires the use of an acid regenerant to neutralize the eluent. Choose the appropriate sulfuric acid concentration as described in Tables 3 or 4, "Matching Regenerant Concentration and Flow Rate to Eluent Concentration and Flow Rate."...

  • Page 27: Operation

    – Operation 3. Operation This section provides instructions for the start-up and operation of the Dionex Chemically Regenerated Suppressor (Dionex CRS 500). The selection and description of each of the suppression modes of operation are covered. 3.1 Chemical Purity Requirements Obtaining precise and accurate results requires eluents that are free of ionic impurities.

  • Page 28: Start Up

    – Operation 3.2 Start Up The Dionex CRS 500 is installed in the column compartment of the chromatography module after the analytical column and before the conductivity detector cell. On all Dionex Ion Chromatography Systems instruments, the Dionex CRS 500 mounts on tabs on the component panel.

  • Page 29: Optimizing Regenerant Concentration And Flowrate

    – Operation 3.3 Optimizing Regenerant Concentration and Flowrate 3.3.1 Anion Regenerant Concentration for Optimized Operation in External Chemical and AutoRegen Modes The Dionex ACRS 500 has the ability to provide continuous suppression of eluents using chemical regeneration with an acid such as sulfuric acid (H ).

  • Page 30: Cation Regenerant Concentration For Optimized Operation For External Chemical And Autoregen Modes

    – Operation For the lower eluent concentration in a given range, choose lower corresponding regenerant concentration; for higher eluent concentration choose higher regenerant concentration. NOTE Most solvents such as methanol have little or no effect on the Anion Regenerant Solution when using an AutoRegen Accessory unit.
  • Page 31 – Operation Table 6 Dionex CCRS 500 2 mm Practical Maximum Eluent Concentration Suppression Guidelines Regenerant Flow Rate = 8 mL/min Maximum Eluent Regenerant Eluent Flow Rate Concentration Concentration Eluent (mL/min) (mM) (mM TBAOH) 0.25 Hydrochloric acid Hydrochloric acid Hydrochloric acid 0.25 Sulfuric Acid 20–25...

  • Page 32: Calculations For Regenerant Concentration

    – Operation For the lower eluent concentration in a given range, choose lower corresponding regenerant concentration; for higher eluent concentration choose higher regenerant concentration. NOTE For the best signal-to-noise ratio and the best overall performance, when using an AutoRegen Cartridge to supply regenerant, Dionex recommends that the Cation Regenerant Solution used with the Dionex CRS 500 be replaced on a regular basis.

  • Page 33: Regenerant Concentration For Dcr Mode

    – Operation B. Dionex Anion Regenerant Concentrate (P/Ns 037164, 039601) should be used to make regenerants for the Dionex Anion Chemically Regenerated Suppressors because of its ease of use and guaranteed high purity. With the DCR Kit, use Anion Regenerant Concentrate 75 mL of 2.0 N Sulfuric Acid (P/N 057559) or the 4-Pak (P/N 057555).

  • Page 34: Troubleshooting Guide

    – Troubleshooting Guide 4. Troubleshooting Guide The purpose of the Troubleshooting Guide is to help you solve operating problems that may arise while using the Dionex Chemically Regenerated Suppressor (Dionex CRS 500). For more information on problems that originate with the Ion Chromatograph or the specific Anion exchange column set in use, refer to the Troubleshooting Guide in the appropriate Installation Manual.

  • Page 35: Drifting Baseline

    – Troubleshooting Guide B. Check for eluent flow out of the suppressor ELUENT OUT port. If there is no flow out of the Dionex CRS 500 ELUENT OUT port, make sure that eluent is entering the suppressor at the ELUENT IN port. If there is no flow at this point, trace the eluent flow path backward through the system to find and remove the blockage.

  • Page 36: Decreased Sensitivity

    – Troubleshooting Guide 4.4 Decreased Sensitivity A. Check for leaks throughout the system. If a fitting is leaking, tighten it carefully until the leak stops. Do not overtighten. If the Dionex CRS 500 is observed to be leaking from the center or bottom seam, see Section 4.6, “Liquid Leaks.” If you cannot cure the problem yourself, call the nearest Dionex Regional Office (see, “Dionex Regional Offices”) for assistance.

  • Page 37: Liquid Leaks

    – Troubleshooting Guide 4.6 Liquid Leaks A. If there is leakage from the ports of the Dionex CRS 500, carefully tighten the fittings in the ELUENT and REGEN IN and OUT ports – be careful not to over-tighten the fittings. If tightening the fittings does not stop the leak, replace the fittings and ferrules. B.

  • Page 38: Suppressor Cleanup

    – Suppressor Cleanup 5. Suppressor Cleanup This section describes routine cleanup procedures for the Dionex Chemically Regenerated Suppressors (Dionex CRS 500) in the case of contamination. Consult the Troubleshooting Guide (see Section 4, “Troubleshooting Guide”) to first determine that the system is operating properly.

  • Page 39: Base-Soluble Contaminants Or Precipitates With The Dionex Crs 500

    – Suppressor Cleanup 5.1 Base-Soluble Contaminants or Precipitates with the Dionex CRS 500 A. Disconnect the analytical (and guard) column(s) from the injection valve and the Dionex CRS 500 Refer to the specific analytical column Product Manual for column cleanup procedures. B.

  • Page 40: Organic Contaminants With The Dionex Acrs 500 Or Dionex Ccrs 500

    – Suppressor Cleanup 5.2 Organic Contaminants with the Dionex ACRS 500 or Dionex CCRS 500 A. Disconnect the analytical (and guard) column(s) from the injection valve and the Dionex CRS 500. Refer to the specific analytical column Product Manual for column cleanup procedures.

This manual is also suitable for:

Dionex crs 500 seriesDionex acrs 500Dionex ccrs 500

Table of Contents