Thermo Scientific Accela Open Autosampler Hardware Manual page 67

Thermo Scientific
4. Slowly rotate the outer knob to adjust the needle penetration depth. The syringe moves
down stepwise into the injection port.
5. When the syringe needle tip enters the valve needle guide, slow down the Z-movement
again. Always observe the syringe needle during this adjustment step.
6. Move down the syringe stepwise until the bottom of the cross bar of the DLW Needle
holder assembly is flush with the lower line of the DLW needle adapter block. See
Figure 45.
7. Press ENTER to save the needle penetration depth value.
8. Verify the defined needle penetration depth value by repeating steps 3 through 6.
Figure 45. Teaching the needle penetration
9. If you hear a click and the ball-spring loaded needle holder is completely compressed,
stop the Z-down movement immediately.
10. Rotate the outer knob stepwise in the opposite direction until the gap of the spring loaded
needle holder is approximately 1 mm, or the bottom of the holder assembly is flush with
the lower surface of the needle adapter block, as in
penetration test.
Figure 46 shows an example of the correct and incorrect positioning of the syringe needle
tip in the valve inlet port.
2
Installing the Accela Open Autosampler
Accela Open Autosampler Object Positions
Figure 45. Repeat and verify the needle
Accela Open Autosampler Hardware Manual 49