System Limitations; Accela Open Autosampler; Sample Volume; Wetted Parts And Material - Thermo Scientific Accela Open Autosampler Hardware Manual

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Reference Information

System Limitations

System Limitations

Accela Open Autosampler

Sample Volume

Wetted Parts and Material

Tubing Internal Diameter (ID) and System Backpressure

Interfacing the Open Autosampler to Other Devices

Synchronization and Output Signals
152 Accela Open Autosampler Hardware Manual
This section contains information about system limitations.
You can operate the DLW Option only with an Accela Open Autosampler, which requires
PAL Firmware level 4.1.x and the APR Control-xt board.
The current version of the DLW Option can handle sample volumes of 2–100 μL.
Some components are not compatible with certain solvents or chemicals that are used in given
applications.
Any tubing and component in the flow path, such as an injection valve, contributes to the
system backpressure. For example, short tubing with an 0.13 mm (0.005 in.) ID can build up
high backpressure when using standard wash pump flow rates.
The wash solvent pumps operate in the range of mL/min. Such a flow rate combined with a
high system backpressure caused by small ID tubing will slow down the pump and, as a result,
the flow rate.
The described situation is only critical if you select an HPLC setup dedicated for nano flow.
This section contains information about interfacing the Open Autosampler to other devices.
Synchronization Signals (Sync Signals) are inputs that tell the Accela Open Autosampler when
to wait or proceed with a sample-processing step. Output Signals (Out Signals) are sent from
the PAL to external devices to indicate status or completion of particular processing steps.
These signals are classified as Objects. Physical Events (for example, TTL-In1) are also
Objects and may be assigned to named signals. PAL Cycles require that certain signals, such as
Start, Inject, and Injected, be defined. For the PAL Events and signal assignments associated
with the standard PAL LC-Inj and LC-Cut cycles, see Table 47.
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