Solvent Delivery Pumps Not Priming; Solvent Delivery Pump Not Functioning - Thermo Scientific Accela Open Autosampler Hardware Manual

Avoiding Trapped Air Bubbles

Solvent Delivery Pumps Not Priming

Solvent Delivery Pump Not Functioning

Thermo Scientific
Figure 61. Ferrule types from various vendors
It is common practice to make a connection only if the ports are wetted. The liquid helps to
prevent trapped air bubbles, which are often tedious to remove or work out of a system.
The solvent delivery pumps of the Pump Module can fail to prime at initial operation.
You must wet the pump at the solvent inlet port or, preferably, prime the tubing from the
Solvent reservoir to the pump and make the connection.
Dry running the pump for a short time will not harm it.
The pump flow rate depends greatly on the viscosity of the selected wash solvent and the
given backpressure of the entire system. Consider the valve bore size (standard Cheminert
valve 0.25 mm), as well as the tubing and injection loop internal diameter.
If in doubt about the delivered volume of a pump, run the following test (Test 1). If the result
of the test is negative, check the flow from the pump directly (Test 2).
 (Test 1) To test the pump flow delivered throughout the entire system
1. Prepare a graduated cylinder with a volume of 10 or 20 mL.
2. Test a solvent, for example, water.
3. Select this path from the local terminal workstation.
Menu > Utilities > Wash Station > selected wash station
The delivery pump and the DLW Actuator are activated, and you can collect the delivered
flow at the DLW syringe needle.
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Troubleshooting Considerations
Accela Open Autosampler Hardware Manual
Troubleshooting
95