Appendix B Creating A Background Plate; Overview; Creating A Background Plate - Applied Biosystems 7300 Installation And Maintenance Manual

Overview

Whenever possible, use a background plate included with the spectral calibration kit.
The plates supplied in the kit contain a buffer that accurately models the reagents used
for PCR, and, therefore, produces high-quality calibration data. However, if a
background plate from a spectral calibration kit is not available, you can create one by
following the procedure below.
Materials
Required

Creating a Background Plate

IMPORTANT!
plate.
Use the plate for background calibration in the same way you use a background plate
from the spectral calibration kit. See
and Optical Calibration."
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Creating a Background Plate
Pipettor, 200-µL Deionized
(with pipette tips) water
Optical Adhesive Cover
Applied Biosystems
Optical 96-Well
Reaction Plate or 96-
Well Fast Reaction
Wear powder-free gloves while creating the background
Remove an Applied Biosystems 96-Well Optical Reaction Plate or 96-Well Fast
1.
Reaction Plate from its box and place it on a clean, dry surface.
Aliquot 50 µL (7300/7500 system) or 20 µL (7500 Fast system) of deionized water
2.
to each well of the reaction plate.
Seal the plate using an optical adhesive cover or optical flat caps.
3.
Safety glasses
Powder-free
or Optical Flat Caps gloves
Chapter 5, "Performing the Background Calibration
Appendix B
B
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