Chapter 6 Performing the Pure Dye Calibration
Evaluating Pure Dye Calibration Spectra
Table 3 Spectra for pure dyes supported by the 7500 Fast system
Instrument
Pure Dye
7500 Fast System
FAM
JOE
NED
ROX
SYBR
Green
Notes
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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Filter
Peak (nm)
A
~520 nm
B
~550 nm
C
~550 nm
D
~610 nm
A
~520 nm