Evaluating Pure Dye Calibration Spectra - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 6 Performing the Pure Dye Calibration

Evaluating Pure Dye Calibration Spectra

Evaluating Pure Dye Calibration Spectra
Table 1 Spectra for pure dyes supported by the 7300 system
Instrument Pure Dye
7300 System FAM
JOE
NED
Notes
80
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Use the tables in this section to verify that the peak for the spectra of the pure dye occurs
at the correct filter for your system:
• 7300 system – see page 80
• 7500 system – see page 82
• 7500 Fast system – see
If the peak for the spectra of the dye occurs in the wrong filter, you may have run the
wrong dye plate during the calibration. Repeat the procedures for only that dye.
Filter Peak (nm)
A ~520 nm
B ~550 nm
C ~550 nm
page 84