Applied Biosystems 7300 Installation And Maintenance Manual page 32

Chapter 1 Before Starting the Installation
Planning the Installation
Recommended Workflow (continued)
7300
~ 01:00:00
7300/7500
~ 02:00:00
See Note below
Note: You must be present at the instrument for approximately 5 minutes of the total run time.
Notes
6
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Time Required
Perform the background calibration (Chapter 5):
a. Prepare the
b. Create a plate document.
~ 00:30:00
c. Perform the background calibration.
d. Analyze the background data.
Perform the optical calibration (7500/7500 Fast systems only,
Chapter 5):
a. Create a plate document.
b. Perform the optical calibration.
~ 00:10:00
c. Analyze the optical calibration data.
Perform the pure dye calibration (Chapter 6):
7500/7500 Fast
a. Prepare the
b. Create a plate document.
c. Perform the
~ 01:20:00
d. Analyze the pure dye calibration data.
e. Evaluate pure dye calibration spectra.
7500 Fast
Verify instrument performance (Chapter 7):
7500 Fast a. Prepare the TaqMan
b. Create a plate document.
c. Perform the
~ 00:40:00
d. Analyze the TaqMan
Task
calibration plate.
background
plates.
pure dye
calibration.
pure dye
®
RNase P verification plate.
.
verification run
®
RNase P plate data.