Applied Biosystems 7300 Installation And Maintenance Manual page 79

Verify that the liquid in each well of the
7.
background plate is at the bottom of the well. If
not, centrifuge the plate again at a higher rpm
and for a longer period of time.
Do not allow the bottom of the
IMPORTANT!
background plate to become dirty. Fluids and
other contaminants that adhere to the bottom of
the plate can contaminate the sample block and
cause an abnormally high background signal.
Continue with "Creating a Plate Document for the
Background Calibration" on page
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 5 Performing the Background Calibration and Optical Calibration
Liquid is at
bottom of well.
54.
Preparing the Background Calibration Plate
Correct
• Not centrifuged with enough force,
or
• Not centrifuged for enough time
Incorrect
53
5