Applied Biosystems 7300 Installation And Maintenance Manual page 66

Chapter 4 Performing the Regions of Interest (ROI) Calibration
Preparing the ROI Calibration Plate
Verify that the liquid in each well of the ROI
3.
plate is at the bottom of the well. If not,
centrifuge the plate again at a higher rpm and for
a longer period of time.
Loading the Plate
Press the tray door to open it.
1.
Load the ROI calibration plate into the plate
2.
holder in the instrument. Ensure that the plate is
properly aligned in the holder.
Load standard plates
(7300/7500 system)
with the notched A12
position at the
top-right of the tray.
Load Fast plates
(7500 Fast system)
with the notched A1
position at the top-left
of the tray.
Close the tray. Apply pressure to the right side of
3.
the tray and at an angle.
Continue with "Creating a Plate Document for the
ROI Calibration" on page
Notes
40
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
12
Notched
corner
Well A1
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
A1
1
A
GR2475
7500
keyed corner
41.
Correct
Liquid is at • Not centrifuged with enough force,
bottom of well. or
• Not centrifuged for enough time
Press
here
Incorrect