Overview; Background Calibration And Optical Calibration - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 5 Performing the Background Calibration and Optical Calibration

Overview

Overview
Time Required
Materials
Required
Applied BiosystemsReal-Time
PCR System Spectral
Calibration Kit
When to Perform
a Background
Calibration
Purpose of the
Background
Calibration
Notes
50
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
You can also use the Real-Time System Setup wizard to perform the procedure in
Note:
this chapter. To access the wizard, select
Fast System Real-Time System Setup Wizard.
• 7300 systems: Background calibration: 30 minutes
• 7500 systems:
– Background calibration: 30 minutes
– Optical calibration: 10 minutes
Background Plate
Perform a background calibration:
• When installing the system (you must perform all calibrations in this order: ROI
background optical (7500/7500 Fast systems only) pure dye instrument
verification).
• Monthly or as often as necessary, depending on instrument use.
• After replacing the lamp.
7500/7500 Fast systems only: You must perform an optical calibration
IMPORTANT!
after every background calibration.
A background calibration measures the level of background fluorescence in the
instrument. During a background calibration run, the system:
• Performs continuous reads of a background plate containing PCR buffer for
10 minutes at 60 °C.
• Averages the spectra recorded during the run and extracts the resulting spectral
component to a calibration file.
Program Files
Safety
Goggles
Powder-free
Gloves
7300/7500/7500
GR2170
Centrifuge with plate
adapter