Applied Biosystems 7300 Installation And Maintenance Manual page 138

Appendix A
Decontaminating the Sample Block
Clean the contaminated wells of the sample
14.
block using a small volume of 95% ethanol
solution:
Pipette a small volume of 95% ethanol
a.
solution into each contaminated well.
In each contaminated well, pipette the
b.
solution up and down several times to rinse
the well.
Pipette the ethanol solution to a waste
c.
beaker.
Repeat steps 8 through 11
15.
the sample block and to verify that you have
eliminated the contamination.
If the contamination is still present, repeat
steps 1 through 7, then go to
Always use deionized water to
IMPORTANT!
rinse wells after cleaning with bleach or ethanol
solution.
Sodium hypochlorite (bleach) is a liquid
disinfectant that can be corrosive to the skin and
can cause skin depigmentation. Read the MSDS,
and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and
powder-free gloves.
Clean the contaminated wells of the sample
16.
block using a small volume of 10% bleach
solution:
Pipette a small volume of 10% bleach
a.
solution into each contaminated well.
In each contaminated well, pipette the
b.
solution up and down several times to rinse
the well.
Pipette the bleach solution to a waste
c.
beaker.
Notes
112
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
to rinse the wells of
step 16.
CHEMICAL HAZARD.
95% ethanol solution