Performing The Background Calibration - Applied Biosystems 7300 Installation And Maintenance Manual

Performing the Background Calibration

Load the plate in the instrument as described in
1.
"Loading the Plate" on page
If you cannot open the tray, the sample
Note:
block may be in its raised position, locking the
tray position. To lower the block, select
Instrument Calibrate, then exit the ROI
Inspector.
In the SDS software:
2.
Select the Instrument tab.
a.
Click
b.
The instrument begins the background
calibration run.
Before starting the run, the instrument
Note:
may pause (up to 10 minutes) to allow the heated
cover to reach the correct temperature.
Continue with "Analyzing the Background
Calibration Data" on page
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 5 Performing the Background Calibration and Optical Calibration
40.
.
56.
Performing the Background Calibration
12
Notched
corner
Well A1
7300/7500 System
Standard plate
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
A1
1
A
GR2475
7500
keyed corner
7500 Fast System
Fast plate
a
b
55
5