Performing The Verification Run - Applied Biosystems 7300 Installation And Maintenance Manual

Performing the Verification Run

Load the plate in the instrument as described in
1.
"Loading the Plate" on page
When closing
Note:
the instrument tray,
apply pressure to the
right side of the tray
and at an angle.
In the plate document:
2.
Select the Instrument tab.
a.
Click
b.
The instrument begins the run, which lasts 1.5 to
2 hours using a 7300/7500 Real-Time PCR
system or <40 minutes using a 7500 Fast
Real-Time PCR system.
Before starting the run, the instrument
Note:
may pause (up to 10 minutes) to allow the heated
cover to reach the correct temperature.
When the run is complete, click
3.
Continue with "Analyzing the TaqMan
Plate Data" on page 94.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
40.
Press
here
.
.
®
RNase P
Chapter 7 Verifying the Instrument Performance
Performing the Verification Run
12
Notched
corner
Well A1
7300/7500 System
Standard plate
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
A1
1
A
GR2475
7500
keyed corner
7500 Fast System
Fast plate
a
b
Click
93
7