Applied Biosystems 7300 Installation And Maintenance Manual page 100

Chapter 6 Performing the Pure Dye Calibration
Preparing Plates and Performing the Pure Dye Calibration
Briefly centrifuge the pure dye plate in a
3.
centrifuge with a plate adapter (<1500xg).
Verify that the dye standard in each well of the
4.
pure dye plate is at the bottom of the well.
If not, centrifuge the plate again at a higher speed
and for a longer period.
Verify that the pure dye plate that you are about
5.
to load matches the dye selected in the Pure
Spectra Calibration Manager.
Load the plate in the instrument as described in
6.
"Loading the Plate" on page
When closing
Note:
the instrument tray,
apply pressure to the
right side of the tray
and at an angle.
Performing the Pure Dye Calibration
In the dialog box that prompts you to load the plate
(see step 1d on page 73, click
the run to complete (~5 minutes).
Notes
74
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
40.
Press
here
, then wait for
<1500× g 00:00:05
Correct
Liquid is at • Not centrifuged with enough force,
bottom of well. or
• Not centrifuged for enough time
12
Notched
corner
Well A1
7300/7500 System
Standard plate
Click
Incorrect
Dye label
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
A1
1
A
GR2475
7500
keyed corner
7500 Fast System
Fast plate
> 00:05:00