Applied Biosystems 7300 Installation And Maintenance Manual page 155

In the SDS software, select
6.
Instrument Calibrate.
In the warning dialog box, click
7.
move the block.
In the ROI Inspector, create the ROI image for
8.
each filter, beginning with Filter A:
In the Exposure Time field, enter 1024.
a.
Click
b.
Select Filter A.
c.
Click
d.
Check the image for saturation.
e.
Normal
No Saturation
Saturation
Good
Record the coordinate of the well that
f.
displays the brightest possible signal
without saturation. This well contains the
best concentration of the custom pure dye
for Filter A.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
to
.
.
Over Saturated
Bad
Appendix C
Determining Optimum Dye Concentration
Select
Click
a
b
c
C
129