Creating A Method - Applied Biosystems 2720 User Manual

Creating a Method

Procedure
To create a method :
Step Action
1 From the Main Menu press F2 (Create).
The Create/Edit screen appears with a default method displayed.
1 Hld 3 Tmp
94.0
5:00
Start
F1
2 Use the arrow keys to move the highlighter to a temperature or time on the graph
and change the parameter.
For example, move the highlighter to the 5:00 time at the left of the screen in the
pre-PCR portion. Press 5 3 0 then press Enter, for 5:30.
3 Make a change to the top line.
For example, use the arrow keys to move the highlighter to the 1 in the upper left
corner of the screen. Press 2, then Enter. Notice that you've added a hold (another
time and temperature segment) to the graph.
4 Press F2 (Store) to save the method.
The Store Method on Instrument screen appears.
Free Mem: 122 methods 946 segments
Accept
F1
The system has given the method a default name of "exp000". We will use a
different name.
5 Press F3 (Method).
The Method Name screen appears.
Method Name exp000
Use ENTER key to select a character.
Accept
F1
6 Press the CE key to clear the field. Then spell out test01 by highlighting each letter
and pressing Enter after each one.
25 Cycles 2 Holds
94.0
72.0
0:30
55.0
0:30
0:30
Store Print
F2 F3
Store Method on Instrument
User: adrian
Method:exp000
User Method
F2 F3
Backsp
F2 F3
72.0
7:00
4.0
More Cancel
F4 F5
Cancel
F4 F5
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Cancel
F4 F5
Getting Started 2-5