Applied Biosystems 7300 Installation And Maintenance Manual page 77

The software then uses the calibration file during subsequent runs to remove the
background fluorescence from the run data.
Fluorescence data collected by the Applied Biosystems 7300/7500/7500 Fast Real-Time
Background
PCR System includes a fluorescent signal inherent to the system, commonly referred to
Fluorescence
as background fluorescence. Background fluorescence is a composite signal found in all
spectral data. This signal consists of fluorescence from several sources, including:
For a new instrument:
Guidelines for
Calibration
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 5 Performing the Background Calibration and Optical Calibration
• Background electronic signal
• Contaminants in the sample block
• The plastic consumable (plates and caps)
• Always start with a new calibration kit.
• Make sure the centrifuge you use is clean. Before centrifuging, wipe down the
bucket using a tissue.
• Handle the calibration plates with care to prevent contamination. Do not place
plates on a lab bench, which may contaminate the plate. Always put calibration
plates back into their original bags.
Overview
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