Applied Biosystems 7300 Installation And Maintenance Manual page 71

Click
5.
The software takes a snapshot, then displays a
message dialog box or an ROI image:
If the software displays:
A very faint ROI image or you cannot generate a
successful calibration.
An acceptable Calibration Image
Successful calibration – All
wells have green circles
Note:
A green circle indicates that the region of interest
for the well position is bright enough.
Click
6.
The software saves the newly generated ROI
calibration for Filter A. "OK" appears next to
Filter A in the Masks Loaded section of the ROI
Inspector window.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 4 Performing the Regions of Interest (ROI) Calibration
.
or
.
Performing the ROI Calibration
Do this:
a. Click .
b. Decrease the value in the Exposure Time field (see
step 1a) by half.
c. Repeat steps 2 and 5.
See "Troubleshooting" on page
Verify that the:
• Wells Found value is 96.
• ROI Image displays a green circle around each well
area.
48.
Successful calibration –
Wells Found must be 96
Click
Displays OK when
save is complete
45
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