Analyzing The Pure Dye Calibration Data - Applied Biosystems 7300 Installation And Maintenance Manual

Analyzing the Pure Dye Calibration Data

When you run each pure dye plate, the SDS Software
automatically creates and saves a plate document for
each dye. After you calibrate all of the pure dye
plates, the plate documents remain open behind the
plate document displayed by the software. To
complete the calibration, analyze all open pure dye
documents as explained below.
From the Windows menu, select the plate
1.
document to analyze.
Select the Results tab, then select the Spectra
2.
tab.
Select all wells of the plate document by clicking
3.
the upper-left corner of the plate grid.
Click (or select Analysis Extract Pure
4.
Spectra).
The SDS software completes the extraction, then
displays a message (see next page).
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 6 Performing the Pure Dye Calibration
Analyzing the Pure Dye Calibration Data
The SDS Software creates a
plate document for each
calibrated dye (behind the
visible plate document)
Select
Select
Click
Click
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