Chapter 5 Performing the Background Calibration and Optical Calibration
Analyzing the Background Calibration Data
Unloading the
Plate
Notes
58
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
PHYSICAL INJURY HAZARD. During instrument operation, the
sample block can be heated to 100 °C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
When the run is complete, remove the background plate:
1.
Press the tray to open it.
a.
Remove the background plate.
b.
Press the tray to move it into the instrument.
c.
Place the background
2.
plate inside its packaging
sleeve, then return it to the
spectral calibration kit in
the freezer.
Do not discard the background plate. If you store the background
IMPORTANT!
plate in its original packaging sleeve, you can use the plate up to three times after
you open it.
Continue with:
• 7300 system –
• 7500/7500 Fast system –
7500/7500 Fast System" on page
"Performing the Pure Dye Calibration" on page
"Performing the Optical Calibration for the
59.
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67.