Overview - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration

Overview

Overview
Time Required
Materials
Required
Applied Biosystems
Real-Time PCR System
Spectral Calibration Kit
When to Perform
the ROI
Calibration
Notes
36
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
You can also use the Real-Time System Setup wizard to perform the procedure in
Note:
this chapter. To access the wizard, select
Fast System Real-Time System Setup Wizard.
30 minutes
ROI Calibration Plate
Perform a regions of interest (ROI) calibration:
• When installing the system (you must perform all calibrations in this order: ROI
background optical (7500/7500 Fast systems only) pure dye instrument
verification).
• Every 6 months, or as often as necessary, depending on instrument use.
• After replacing the lamp.
After every ROI calibration, you must perform a background calibration,
IMPORTANT!
optical calibration (7500/7500 Fast systems only), pure dye calibration, and instrument
verification.
Program Files
Safety
Goggles
Powder-free
Gloves
7300/7500/7500
GR2170
Centrifuge with plate
adapter