Applied Biosystems 7300 Installation And Maintenance Manual page 122

Chapter 7 Verifying the Instrument Performance
Analyzing the TaqMan
In the Well Inspector, select Omit Well.
c.
Click (or select Analysis Analyze) to
d.
reanalyze the run without the outlying data.
Repeat step 7 on the previous page for other
8.
wells with outlying data.
Up to six wells from each
IMPORTANT!
replicate group in a RNase P plate can be omitted
to meet specifications. If the analysis contains
more than six outliers, troubleshoot the RNase P
verification run. See "Troubleshooting" on
page 99.
In the Results tab, select the Standard Curve
9.
tab.
In the Standard Curve tab, verify that the R2
10.
value is ≥0.990.
In the Results tab, select the Report tab.
11.
Notes
96
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
®
RNase P Plate Data
Select
Omitted well
is crossed-out
Select
Must be ≥0.99
Select