Applied Biosystems 7300 Installation And Maintenance Manual page 29

You can also use the Real-Time System Setup wizard after the system is up and running,
Using the Wizard
to perform the routine recalibrations and verification runs that ensure optimum
to Recalibrate the
performance:
System
Recalibration/
Verification
ROI Defines the well positions on the sample block.
Background Measures the level of background fluorescence in the
instrument. During a run, the software removes the
background fluorescence from the run data.
Optical Compensates for the physical effects of the additional
(7500/7500 Fast filter present in 7500/7500 Fast instruments.
systems only)
Pure Dye Characterizes each dye. During a run, the software
uses the pure dye calibration spectra to distinguish the
individual contribution of each dye in the collective
fluorescence gathered by the instrument.
Verifies that the instrument can distinguish between
RNase P
5,000 and 10,000 genome equivalents of the RNase P
gene with a 99.7% confidence level.
Access the wizard by selecting
System
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
• Performing pure dye calibrations
• Verifying instrument performance
Purpose
Real-Time System Setup Wizard.
Chapter 1 Before Starting the Installation
Selecting the Installation Method
When to Perform
• Every 6 months
• After replacing the halogen lamp
After an ROI recalibration, you must also
perform a background, optical (7500/7500
Fast systems only), pure dye calibration, and
instrument verification.
• Once a month
• After replacing the halogen lamp
After a background recalibration, you must
perform an optical (7500/7500 Fast systems
only).
• Once a month
• After performing a background calibration
• After replacing the halogen lamp
Every 6 months
Before performing, you must perform a
background calibration (7300 systems) or
optical calibration (7500/7500 Fast systems);
After moving the instrument to another
location or as needed to verify the function of
the instrument
Program Files 7300/7500/7500 Fast
1
3