Overview; Chapter 6 Performing The Pure Dye Calibration - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 6 Performing the Pure Dye Calibration

Overview

Overview
Time Required
Materials
Required
Applied Biosystems
Real-Time PCR System
Spectral Calibration Kit
When to Perform
Pure Dye
Calibrations
Notes
68
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
You can also use the Real-Time System Setup wizard to perform the procedure in
Note:
this chapter. To access the wizard, select
Fast System Real-Time System Setup Wizard.
• 7300 systems: 45 minutes
• 7500/7500 Fast systems: 1 hour
7300, 7500/7500
Fast system
Pure Dye Plates
™ ™ ™
(FAM , JOE , NED ,
™ ®
ROX , SYBR Green,
™ ®
TAMRA, and VIC dyes)
Perform a pure dye calibration:
• When installing the system (you must perform all calibrations in this order: ROI
background optical (7500/7500 Fast systems only) pure dye instrument
verification).
• Every 6 months, depending on instrument use.
You must perform a background run before every pure dye calibration.
IMPORTANT!
Because the age and use of instrument components can affect pure spectra readings,
Applied Biosystems recommends performing a pure dye calibration at least every six
months.
Program Files
7500/7500 Fast
system only
Safety
Glasses
Pure Dye Plates
(CY3, CY5, and Powder-free
®
TEXAS RED
Gloves
dyes)
7300/7500/7500
GR2170
Centrifuge with plate
adapter