Applied Biosystems 7300 Installation And Maintenance Manual page 86

Chapter 5 Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
Allow the ROI calibration plate to warm to room
3.
temperature (approximately 5 minutes).
Do not remove an ROI calibration
IMPORTANT!
plate from its packaging until you are ready to
run it. The fluorescent dye in the wells of the
plate is photosensitive. Prolonged exposure to
light can diminish the fluorescence signal
strength of the plate.
Remove the ROI calibration plate from its
4.
packaging.
Do not discard the packaging for
IMPORTANT!
the ROI calibration plate. The plate can be used
up to three times if it is stored in its original
packaging sleeve.
Standard plates only: Vortex the plate for
5.
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
Briefly centrifuge the ROI calibration plate in a
6.
centrifuge with a plate adapter (<1500xg).
Verify that the liquid in each well of the
7.
background plate is positioned at the bottom of
the well. If not, centrifuge the plate again at a
higher rpm and for a longer period of time.
Notes
60
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Ambient >00:05:00
<1500× g 00:00:05
Correct
Liquid is • Not centrifuged with enough force,
positioned at or
bottom of well.
• Not centrifuged for enough time
Incorrect