Applied Biosystems 7300 Installation And Maintenance Manual page 63

A regions of interest (ROI) calibration maps the positions of the wells on the sample
Purpose of the
block so that the software can associate increases in fluorescence during a run with
ROI Calibration
specific wells of the plate. Because the instrument uses a set of optic filters to distinguish
the fluorescence emissions gathered during runs, you must generate a calibration image
for each individual filter to account for minor differences in the optical path.
ROI calibration image
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 4 Performing the Regions of Interest (ROI) Calibration
Overview
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