Applied Biosystems 7300 Installation And Maintenance Manual page 89

Real-time pcr system
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Analyzing the Optical Calibration Data
Unloading the
sample block can be heated to 100 °C. Before performing the following procedure, be
Plate
sure to wait until the sample block reaches room temperature.
Continue with
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 5 Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
In the SDS software, click
1.
The software extracts the optical calibration, then displays a message indicating the
extraction is complete.
Click
2.
.
In the SDS software, select File Close.
3.
PHYSICAL INJURY HAZARD. During instrument operation, the
Remove the ROI calibration plate:
1.
Press the tray to open it.
a.
Remove the ROI calibration plate.
b.
Press the tray to move it into the instrument.
c.
Place the ROI calibration
2.
plate inside its packaging
sleeve, then return it to the
spectral calibration kit in
the freezer.
Do not discard the ROI calibration plate. If you store the plate in its
IMPORTANT!
original packaging sleeve, you can use the plate up to three times after you open it.
"Performing the Pure Dye Calibration" on page
(or select Analysis Extract).
-20 to -
67.
63
5

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