Appendix A Creating Detectors - Applied Biosystems 7300 Getting Started Manual

Appendix A Creating Detectors

Sample Experiment
In the example plus/minus experiment, a detector was created for the E. coli target, and another was created for the IPC.
The E. coli detector was assigned a blue color and IPC a black color.
Notes
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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Plus/Minus Assay Getting Started Guide
In the Reporter Dye and Quencher Dye drop-down lists, select the appropriate dyes
5.
for the detector.
The dyes that appear in the Reporter and Quencher Dye lists are those that
Note:
have been previously entered using the Dye Manager. If the dye that you want to
use does not appear in a list, use the Dye Manager to add the dye and then return to
step 5 in this procedure. Refer to the Online Help for more information.
Click the Color box, select a color to represent the detector using the Color dialog
6.
box, then click OK.
Optionally, click the Notes field, then enter any additional comments for the
7.
detector.
Click OK to save the detector and return to the Detector Manager.
8.
Repeat steps 2 to 8 for the remaining detectors.
9.
In the Detector Manager, click Done when you finish adding detectors.
10.
When creating detectors, you use the reporter dye and quencher information (and
Note:
optionally, the gene name or symbol for the sample name). You can view the contents in
a spreadsheet program, such as Microsoft
®
Excel.